Doxorubicin (Adriamycin, NSC 123127, DOX) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage, mitophagy and apoptosis in tumor cells. • You may need to download version 2.0 now from the Chrome Web Store. Parasites were metabolically labeled with [3H]hypoxanthine, treated with etoposide (■), gatifloxacin (●), levofloxacin(▲), ciprofloxacin (□), fleroxacin (○), or nalidixic acid (△) at the indicated concentrations for 18-22 h, lysed with SDS and digested with RNases. Mitoxantrone is an inhibitor of type II topoisomerase and protein kinase C (PKC) with IC50 of 8.5 μM for PKC. Phenoxodiol also inhibits DNA topoisomerase II. See the answer. Growth inhibitory effects of Epirubicin human pancreatic cancer cells. Nalidixic acid (NSC-82174) is a synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. Cell viability and caspase 3/7 activity in MDA-MB-231 cells co-treated with carbenoxolone, novobiocin, ibrutinib and bisphosphonates. If you are at an office or shared network, you can ask the network administrator to run a scan across the network looking for misconfigured or infected devices. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. A black female mouse was crossed with a black male mouse, 3 of their 10 offspring were white. Coefficient of variation for each data point was 19%. Immunohistochemistry and quantification of cleaved caspase 3 expression in DU145-DR tumor xenografts from (D). In addition, during replication, the newly replicated duplex of DNA and the original duplex of DNA become intertwined and need to be completely separated in order to ensure genomic integrity as a cell divides. Examples of type IIB topoisomerase include topo VI. Cell viability 72 hours after treatment was determined by AlamarBlue assay (Invitrogen) according to manufacturer's instructions. It is also split into two subclasses: type IIA and type IIB topoisomerases, which share similar structure and mechanisms. Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. When outside of replication or transcription, DNA needs to be kept as compact as possible and these three states help this cause. All Rights Reserved. It has selective inhibitory effect on the phosphorylation of histone H1 and H3, but less effect on other histones. 10-Hydroxycamptothecin is a cell-permeable powerful DNA topoisomerase I inhibitor. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Camptothecin (NSC-100880, CPT) is a specific inhibitor of DNA topoisomerase I (Topo I) with IC50 of 0.68 μM in a cell-free assay. Lower class women tend to be more of a survivor when it comes to living without money and would tend to accept you for who you are and not what you have. Not for human use. This problem has been solved! Expert Answer 100% (1 rating) Clinafloxacin (CI-960, PD127391, AM-1091) is a fluoroquinolone that inhibits both DNA gyrase and topoisomerase IV dually in Streptococcus pneumoniae. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide. • Betulinic acid (ALS-357), a pentacyclic triterpenoid from Syzigium claviflorum, is a inhibitor of HIV-1 with EC50 of 1.4 μ M. Betulinic acid acts as a new activator of NF-kB.Phase 1/2. Ciprofoxacin (Cipro) reduces cancer cell survival in combination with chemotherapy. Type I topoisomerase cuts one strand of a DNA double helix and then reanneals the cut strand. If energy cannot be created or destroyed why does the earth need a constant supply of energy from the sun? It is a weak topoisomerase II inhibitor and forms stable DNA adducts through alkylation with specificity for DNA hypermethylated sites. Berberine chloride is a dual topoisomerase I and II inhibitor. Another way to prevent getting this page in the future is to use Privacy Pass.